See Also: Herbal medicine (botanical medicine, herbology, phytomedicine)(health)
reap(dictionary)
reap(2)(dictionary)
reap(4)(dictionary)
reap(3)(dictionary)
reap(1)(dictionary)
Reap(medicine)
Siem Reap Airways(tourism)
Siem Reap Airways flights(tourism)
Siem Reap Airways Intl(tourism)

nick translation (medicine) and reap (oh)


nick translation (medicine)


nick translation
<molecular biology, technique> A technique used to radioactively label DNA.

E. Coli DNA polymerase I will add a nucleotide, copying the complementary strand, to the free 3' OH group at a nick, at the same time its exonuclease activity removes the 5' terminus. The enzyme then adds a nucleotide at the new 3' OH and removes the new 5' terminus. In this way one strand of the DNA is replaced starting at a nick, which effectively moves along the strand. Nick translation refers to this translation or movement and not to protein synthesis.

In practice, DNA is mixed with trace amounts of DNAase I to generate nicks, DNA polymerase I and labelled nucleotides. Because the nicks are generated randomly the DNA preparation can be uniformly labelled and to a high degree of specific activity.


reap (oh)



[Language: Old English; Origin: reopan]
[T] to get something, especially something good, as a result of what you have done
reap the benefit/reward/profit (of sth)
::Those who do take risks often reap the rewards.
you reap what you sow
used to say that if you do bad things, bad things will happen to you, and if you do good things, good things will happen to you
[I and T] old-fashioned to cut and collect a crop of grain
-see also harvest harvest
-- reaper n [C]
-see also Grim Reaper